Tuesday October 25th
11:30 am - 13:00 pm

The journey of mRNA from the chromatin to the nuclear pore complex

Conférence en English

Our lab has been interested for many years in the process and regulation of mRNA biogenesis, surveillance and transport through nuclear pores using the yeast S. cerevisiae as a model system. Over the past few years, we proposed and demonstrated that posttranslational modifications, and in particular ubiquitylation, provides a molecular support to insure the correct chronology of events resulting in the formation of a mature and export-competent mRNA. In this respect, we could recently show the role of some histone marks (and in particular ubiquitylation of the histone H2B) in the control of mRNA splicing and nuclear export. We are now deciphering more deeply how chromatin dynamics (histone marks and chromatin remodellers) can participate to mRNP formation. Ubiquitylation processes also regulate dissociation of some processing factors prior transport to the cytoplasm and thereby participates to the quality control of mRNPs prior their export out of the nucleus. Our objective is now to use recent technological development or adaptations of super resolution microscopy to dissect the spatiotemporal coordinated cascade leading mRNPs from their site of transcription to their site of nuclear exit.