This course will explore the versatility of non-genic DNA elements and non-coding RNAs across a spectrum of cellular processes, in humans and model organisms, and their implication in physiology and disease, expanding the topics around the fields of genomics, epigenetics and transcriptomics including single-cell technologies and analysis, epigenome and gene expression regulation, genome organisation and cell clonality. . Internationally recognized experts will present their latest findings related to the identification and functional characterization of the noncoding genome and discuss novel concepts in genome regulation and evolution, with a strong emphasis on experimental and computational tools. Thematic sessions will include computational analysis of cell heterogeneity, methods for assessing cell heterogeneity and plasticity, epigenome in regulation of gene expression, retroelements in cell plasticity, Dark genome in cell identity and clonality, and spatial organization. This course will offer to young students and research fellows the opportunity to broaden their knowledge and discuss their work with an international scientific community in a warm and stimulating environment at the Institut Curie in Paris.